Given its importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. Characteristics of a cloning vectors. This factor is relevant for both cloning and expression vectors. In physics, when you break a vector into its parts, those parts are called its components.For example, in the vector (4, 1), the x-axis (horizontal) component is 4, and the y-axis (vertical) component is 1.Typically, a physics problem gives you an angle and a magnitude to define a vector; you have to find the components yourself using a little trigonometry. A cloning vector should possess an origin of replication so that it can self-replicate inside the host cell. PCR cloning differs from traditional cloning in that the DNA fragment of interest, and even the vector, can be amplified by the Polymerase Chain Reaction (PCR) and ligated together, without the use of restriction enzymes. By Steven Holzner . 7. Cloning vectors in yeast include yeast artificial chromosomes (YACs). • The insertion of the fragment is carried out by treating the vector and the foreign DNA with a restriction enzyme that creates the same overhang, then ligating the fragments together. Based on the naturally occurring f factor plasmid found in the bacterium E.coli. An expression vector has features that any vector may have, such as an origin of replication, a selectable marker, and a suitable site for the insertion of a gene like the multiple cloning site.The cloned gene may be transferred from a specialized cloning vector to an expression vector, although it is possible to clone directly into an expression vector. Genetic engineering is used in thousands of laboratories around the world. It should have a restriction site for the insertion of the target DNA. • A small piece of DNA into which a foreign DNA fragment can be inserted. It should have a selectable marker with an antibiotic resistance gene that facilitates screening of the recombinant organism. Cloning Vector • It is the central component of a gene cloning process. Elements. Shuttle vector Cloning vector is a small DNA molecule capable of self-replication inside the host cell. it must be small in size; A similar vector type is the transcription-only vector that goes only to the mRNA production phase and requires less components than expression vectors. A cloning vector is a small piece of DNA, taken from a virus, a plasmid, or the cell of a higher organism, that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloningpurposes. Cloning vector. Vector Size . Selecting the Cloning System and Plasmid Vector. YAC (Yeast Artificial Chromosome) : A vector of hundreds to kilobases long used for cloning … Cloning vector-characteristics and types Cloning vector. The only aspect to consider here is whether you are cloning … BAC (Bacterial Artificial Chromosome): A vector used to clone DNA fragments of 100-300 kb insert size in E.coli cells. PCR cloning is a rapid method for cloning genes, and is often used for projects that require higher throughput than traditional cloning methods can accommodate. Cloning vector is used for replicating donor DNA fragment within host cell.